COVID-19 SOPs and protocols

RESOURCES & TOOLS

Immunomonitoring for COVID-19 clinical trials

SOPs were developed for the collection of samples obtained from subjects in COVID-19 vaccine clinical trials, and subsequent analysis by laboratories within the network. These SOPs are available for use by the scientific community.

Sample handling and preparation SOPs

Collection and Handling of Biological Material ⬇

Sample Handling ⬇

Preparation of PBMCs ⬇

Whole Blood Processing ⬇

Salivary Collection ⬇

Experimental SOPs

- VACCELERATE Network -

Flow-based Neutralisation Assay ⬇
- Quantification and comparison of the neutralization capacity of plasma/serum samples from convalescent or vaccinated human subjects against wild type and VoCs SARS-CoV-2

Meso Scale Discovery (MSD) Assay ⬇
- Quantification and comparison of neutralization capacity of plasma/serum samples from convalescent or vaccinated subjects against SARS-CoV-2

Haemagglutination Assay ⬇
- Detection of SARS-CoV-2 antibodies binding to the viral receptor binding domain (RBD) in serum

Cellular Immunity Assay ⬇
- qPCR-based detection of CXCL10 in lithium heparin whole-blood samples

Salivary Antibody Assay ⬇
- MSD-based assay for the quantification of antibodies specific to SARS-CoV-2 spike proteins in salivary samples

- CEPI Network -

ELISA ⬇
- Enzyme-linked immunosorbent assay for quantification of SARS-CoV-2 Spike, RBD, of Nucleocapside specific IgG antibodies in human serum samples

ELISPOT ⬇
- Enzyme-linked immunosorbent spot assay for quantifying the response of peripheral blood mononuclear cells (PBMCs) to SARS-CoV-2 Spike protein antigens

Wild-type virus neutralisation ⬇
- Determination of the SARS-CoV-2 neutralisation capacity of serum samples based on microplaque quantification

Contact details:

contact.us@euvaccine.eu

Acknowledgment:

VACCELERATE project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 101037867. This communication reflects the views only of the author, and the European Union cannot be held responsible for any use which may be made of the information contained therein.