Malaria SOPs, assays and reagents

RESOURCES & TOOLS

Malaria protocols, assays and guidance reagents

Malaria research reference reagents, guidance documents for antibody-dependent cellular parasite inhibition (ADCI), intracellular Cytokine Staining (ICS) and ELISPOT, and SOPs for IFA have been developed to facilitate consistent, reproducible and comparable intra- and inter-lab performance and increased accuracy and precision of assay data. These are available to the scientific community.

Experimental SOPs

Routine Culturing of Plasmodium falciparum ⬇

Growth Inhibition Assay (GIA) with Lactate Dehydrogenase (LDH)⬇
- Quantification of IgG’s obtained from patients or animals in their ability to inhibit invasion or intraerythrocytic development of P. falciparum into erythrocytes. The read-out system is detection of pLDH (plasmodium Lactate DeHydrogenase).

Anti-AMA-1 IgG (BG98) SOP ⬇
- Rabbit malaria-immune Ig for Growth Inhibition Assay (GIA) standardisation.

Antigen Reversal Growth Inhibition Assay (GIA) with pLDH read-out ⬇
- Study of the specificities responsible for growth inhibition in normal GIAs.

Enzyme-Linked Immunosorbent Assay (ELISA) SOP ⬇

Avidity ELISA ⬇
- Quantification of the avidity of a polyclonal antiserum to a specific antigen using sodiumthiocyanate (NaSCN). The avidity-index is the NaSCN-concentration at which 50% of the bound antibodies is eluted off.

Competition ELISA ⬇
- To demonstrate the antigen specificities of malarial antibodies in immune serum or plasma by direct competition with different allelic forms of the homologous antigen.

Measles ELISA ⬇
- Detection of antibodies (Ab) against Measles virus in the serum of Resus monkeys by a sandwich Enzyme Linked ImmunoSorbent Assay.

Immuno-Fluorescence Assay (IFA) SOP ⬇

Research Reagent Repository

Research Reagent Repository may be accessed by following the link www.malariaresearch.eu

Related resources

Check Tools for Data Management and Analysis, including GIA data analysis and management application and auditable data Management System for Elisa.

Contact details:

contact.us@euvaccine.eu

Acknowledgment:

This project has received funding from the European Union’s FP7-HEALTH research and innovation programme under grant agreement No 223126. This communication reflects the views only of the author, and the European Union cannot be held responsible for any use which may be made of the information contained therein.